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Usbio/M2155-08A-Biotin Mouse Anti-Maltose Binding Protein (Biotin)/M2155-08A-Biotin/100ul
Maltosebindingproteinisabacterialprotein,whichisoftenusedinproteinexpressionstudiesbecauseitcreatesastablefusionproductthatdoesnotappeartointerferewiththebioactivityoftheproteinofinterest.Italsoallowsforitseasypurificationfrombacterialextractsundermildconditions.Proteintagginghasbecomeawidelyappliedmethodforreadypurificationofproteins.Thespecificinteractionbetweenthetagandasolid-phaseligandallowsfortheselectivebindingandelutionofthetaggedprotein.Manyvectorsthatincorporatethesetagshavebeenengineeredwithacleavagesitebetweenthetagandtheproteinofinterest.Thispermitstheseparationoftheproteinfromthetag,ifneeded.Onecommonlyusedsystemisthemaltose-bindingprotein(MBP).Inthissystem,thetargetgeneisinsertedintheappropriatevectorandexpressed.Followingpurificationusinganamylosecolumn,theMBP-targetproteincomplexcanbeelutedfromthecolumnwithmaltoseandcleavedwithaspecificproteasetoyieldthepurifiedtargetprotein.