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Usbio/036575-ML750 Rabbit Anti-His-Tag (Poly-His, Hexa-HIS, Hexa-Histidine) (MaxLight 750)/036575-ML750/100ul
Usbio/036575-ML750 Rabbit Anti-His-Tag (Poly-His, Hexa-HIS, Hexa-Histidine) (MaxLight 750)/036575-ML750/100ul
商品编号：
036575-ML750
品牌：
USbiological
市场价：
¥14220.00
美元价：
6043.50
产品分类：
其他
公司分类：
Immunological_reagent
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
MaxLight™750isanewNearIRstabledyeconjugatecomparabletoDyLight™750,AlexaFluor™700andoffersbetterlabelingefficiency,brighterimagingandincreasedimmunodetection.Absorbance(759nm);Emission(780nm);ExtinctionCoefficient240,000.
Epitopetagsconsistingofshortsequencesrecognizedbywell-characterizedmonoclonalantibodieshavebeenwidelyusedinthestudyofproteinexpressioninvarioussystems.The6xHIStag(HHHHHH),recognizedbythemonoclonalantibodyclone6AT18providesanestablishedexampleofthisapplication.6xHIS-taggedfusionproteinsareeasilypurifiedfromcelllysatesbyaffinitychromatographyusingNickel-Sepharoseresin.
Applications
SuitableforuseinWesternBlotandFLISA.Otherapplicationshavenotbeentested.
RecommendedDilution
WesternBlot:1:1000Optimaldilutionstobedeterminedbytheresearcher.
StorageandStABIlity
Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
Note:Applicationsarebasedonunconjugatedantibody.
References
1.Hochuli,E.,Doebeli,H.,andSchacher,A.,Newmetalchelateabsorbentselectiveforproteinsandpeptidescontainingneighboringhistidineresidues.J.Chromatogr.411:177-184(1987).品牌介绍
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