Usbio/I1904-83P1小鼠抗IgG2a小鼠阴性同型对照/I1904-83P1/100试验
商品编号：
I1904-83P1
品牌：
USbiological
市场价：
¥9200.00
美元价：
3910.00
产品分类：
其他
公司分类：
Immunological_reagent
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IsotypeControls
Isotypecontrolsareatypeofnegativecontroldesignedtomeasurethelevelofnon-specificbackgroundsignalcausedbyprimaryantibodies, baseduponthetissuetypeofthesample. Non-specificbindingofprimaryantibodiesaretypicallycausedbythefollowingreasons
Usually,thebackgroundsignalistheresultofimmunoglobulinsbindingnon-specificallytoFcreceptorspresentonthecellsurface.Forexample, antibodiesraisedinmice,particularlythoseoftheIgG2aisotype,willbindstronglytosomehumanleukocytesregardlessofthetestantibody specificity.InthiscaseyouwouldneedamouseIgG2aisotypecontrolforusewithhumancellsortissues.Isotypecontrolsaremostcommonly usedinflowcytometryexperiments,butmayalsobeusedinimmunohistochemistry.
SelectinganIsotypeControl
Typically,anisotypecontrolismatchedtothehostspeciesandisotypeofyourspecificprimaryantibody,the heavychain (IgA,IgG,IgD,IgE,orIgM), subclass and lightchain (kappa,lamBDa)classoftheprimaryantibody; thesameFluorochrome(PE,APC,etc.),andhavethesameF:Pratio.F:Pisameasurementofhowmanyfluorescentmoleculesare presentoneachantibody.
Whenusingdirectlylabeledprimaryantibodies,itisalsonecessarytomakesurethattheisotypecontrolisconjugatedtothesamefluorochrome orlabelasthetestantibody.Itisnotsufficienttouseonewithaspectrallysimilarfluorochrome.
Non-specificBinding
Toavoidnon-specificbinding,youalsoneedtoblockFcreceptorsoncelltypes,suchasspleencells,withFcRblockingreagents.Isotypecontrolsshouldbeusedatthesameconcentration/dilutionastheprimaryantibody.Isotypecontrolsaremainlyusedtodetermineifthestainingisreal.Apartfromisotypecontrols,unstainedcellsshouldalwaysbeincludedinthe experimentalset-uptomonitorautofluorescence.
ApplicationsforIsotypeControls
Althoughisotypecontrolsaremainlyusedinflowcytometry,theycanbeusedasstandardblockingagentsandproteincoatingagentsforotherapplicationsincluding immunofluorescence,immunocytochemistry,westernblotting,andELISA.
Recognizesnon-specifcbindingofmousemonoclonalantibodies,isotypeIgG2atohumantissue.RecognizesaratcellsurfaceMarkerandthereforecannotbeusedasanegativecontrolinthisspecies.|Alsosuitableasanegativecontrolwithbovine,sheep,porcine,equine,canine,rabbitandguineapigtissues.
Applications
SuitableforuseinFlowCytometry.Otherapplicationsnottested.
RecommendedDilution
FlowCytometry:Titratetoaconcentrationequaltothatofthetestreagents.10ullabels10e6cellsin100ul.Optimaldilutionstobedeterminedbytheresearcher.
StorageandStABIlity
Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestableforatleast12months.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
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