Usbio/G8965-01D小鼠抗绿色荧光蛋白（GFP）/G8965-01D/100ug
商品编号：
G8965-01D
品牌：
USbiological
市场价：
¥13280.00
美元价：
5644.00
产品分类：
其他
公司分类：
Immunological_reagent
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Thegreenfluorescentprotein(GFP),originallydiscoveredinthejellyfish,Aequoreavictoria,iscomposedof238aminoacidsandhasamolecularweightof27kD.GFPisintheshapeofacylinder,comprising11strandsofbeta-sheetoutside,asinglealphahelicalstrandcontainingthechromophorerunningthroughthecenter,andshorthelicalsegmentsontheends.GFPtransducesthebluechemiluminescenceoftheproteinaequorinintogreenfluorescentlightbyenergytransfer.GFPhasbeenwidelyusedasatracerinvariousdiciplinesofBIOLOGyresearch,suchasfluorescencemicroscopy,reportergeneexpression,andinvivoimaging.LaboratorymutationofGFPgeneratedthewidelyusedanalogGFPuv,whichhasbrighterfluorescenceandmoredesirableexpressionproperties.
Applications
SuitableforuseinELISA,WesternBlotandImmunoprecipitation.Otherapplicationsnottested.
RecommendedDilution
DirectELISA:0.5-1ug/mlDetectionlimitforrGFPuv~1ng/well.WesternBlot:1-2ug/mlDetectionlimitforrGFPuv~5ng/laneundernon-reducingandreducingconditions.Immunoprecipitation:1ug/mlOptimaldilutionstobedeterminedbytheresearcher.
StorageandStABIlity
Lyophilizedpowdermaybestoredat-20°C.Reconstitutetonominalvolumebyaddingsterile40-50%glycerol,PBS.Aliquotandstoreat-20°C.Reconstitutedproductisstablefor12monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.
品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
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