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Usbio/H1847-52X-ML550兔抗血凝素标签（CYPYDVPDYASL）（HA标签）（BSA&；无叠氮化物（MaxLight 550）/H1847-52X-ML550/100ul
Usbio/H1847-52X-ML550兔抗血凝素标签（CYPYDVPDYASL）（HA标签）（BSA&；无叠氮化物（MaxLight 550）/H1847-52X-ML550/100ul
商品编号：
H1847-52X-ML550
品牌：
USbiological
市场价：
¥16980.00
美元价：
7216.50
产品分类：
其他
公司分类：
Immunological_reagent
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
MaxLight™550isanewYellow-GreenphotostabledyeconjugatecomparabletoAlexaFluor™546,555,DyLight™549,Cy3™,TRITCandoffersbetterlabelingefficiency,brighterimagingandincreasedimmunodetection.Absorbance(550nm);Emission(575nm);ExtinctionCoefficient150,000.
Asmallsegmentoftheviralhemagglutinincoatproteiniswidelyusedasanepitopetagtofacilitatepurificationandfunctionalanalysisofproteinsofinterest.EpitopetagsareshortpeptidesequencesthatareeasilyrecognizedbyreADIlyavailabletag-specificantibodies.Ligationofepitope-tagencodingDNAtoclonedDNAsofinterestproducesepitopetaggedfusionproteinsthatcanthenbesubjectedtoantibody-dependentexperimentalprocedures.Epitopetaggingtechniquesarewidelyusedinthepurification,identification,andfunctionalanalysisofproteins.Expressionvectorsforproducingtagfusionproteinsinavarietyoforganisms(includingbacteria,yeast,insect,andmammaliancells)arecommerciallyavailable.Someofthemorecommonlyusedfusiontagsinclude:c-myc,GFP,GST,HA,His,amdMBP.
Applications
SuitableforuseinWesternBlotandImmunoprecipitation.Otherapplicationsnottested
RecommendedDilutions
WesternBlot:5-10ug/mlfor2hoursatRT.Immunoprecipitation:10ug/mgproteinlysate.UseProteinA.Optimaldilutionstobedeterminedbytheresearcher.
PositiveControl:|ProteinsdisplayingtheYPYDVPDYAHA-tag
StorageandStABIlity
Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12months.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
Note:Applicationsarebasedonunconjugatedantibody.
References
1.Terpe,K.,Appl.Microbiol.Biotechnol.60:523-533(2003).2.Tu,Y.,etal.,Mol.Biol.Cell9:3367-3382(1998).3.BhattacharyaandCabral,Mol.Biol.Cell15(7):3123-3131(2004).4.Yamanaka,etal.,Curr.Biol.13(9):734-743(2003).5.Chang,etal.,GenesDev.18(16):2010-2023(2004).6.Munro,S.,Pelham,H.R.,EMBOJ.3:3087-3093(1984).7.Madeo,F.,etal.,Mol.Cell9:911-917(2002).8.Ruckdeschel,K.,etal.,J.Immunol.168:4601-4611(2002).9.Field,J.,etal.,Mol.Cell.Biol.8:2159-2165(1988).10.Schirmer,E.,etal.,J.CellBiol.153:479-490(2001).11.Bjerling,P.,etal.,Mol.Cell.Biol.8:2170-2181(2002).12.Wilson,l.A.,etal.,Cell37:767-78(1984).13.Muster,T.,etal.,J.Virol.69:6678-6686(1995).品牌介绍
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