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Usbio/M9760-01A-Biotin Mouse Anti-Myeloperoxidase (MPO, PANCA) (Biotin)/M9760-01A-Biotin/100ul
Usbio/M9760-01A-Biotin Mouse Anti-Myeloperoxidase (MPO, PANCA) (Biotin)/M9760-01A-Biotin/100ul
商品编号：
M9760-01A-Biotin
品牌：
USbiological
市场价：
¥15760.00
美元价：
6698.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
MPOisanimportantcomponentofazurophilicgranulesinneutrophils,beinginvolvedinmicrobicidalprocesses.Theproteinisamultimerof2heavychains(55kD)andtwolightchains(15kD),theheavychainsbeinglinkedbyadisulfidebond.
Applications
SuitableforuseinFlowCytometry,Immunohistochemistry,ELISAandWesternBlot.Otherapplicationsnottested.
RecommendedDilution
FlowCytometry:1:50-1:100.MembranepermeABIlizationisrequired.Immunohistochemistry(Frozen):1:1000-1:5000Immunohistochemistry(Paraffin):1:500-1:1000WesternBlot:Non-reducingconditions.Optimalworkingdilutionstobedeterminedbyresearcher.
RecommendedSecondaryReagent(FC):|I1904-66A:IgG,F(ab’)2(FITC)PabRbxMo||RecommendedNegativeControl
I1904-78R:IgG1MurineNegativeControl
RecommendedPositiveControlTissue(IHC)
Bonemarrow
RecommendedSecondaryAntibodies(IHC)
I1904-65W:IgG,F(ab’)2(HRP)PabRbxMoI1904-66C:IgG,F(ab’)2,X-Adsorbed(HRP)PabRbxMo
Hybridoma
SpleencellsfromimmunizedmicewerefusedwithcellsofthemouseX63AG8-653myelomacellline.
StorageandStability
Maybestoredat4°Cforshort-termonly.Forlong-termstorage,storeat-20°C.Aliquotsarestableforatleast12monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.
Note:Applicationsarebasedonunconjugatedantibody.
Immunogen
HumanMyeloperoxidase.
Form
SuppliedasaliquidinPBS,pH7.2.LabeledwithBiotin.
Purity
PurifiedbyProteinGaffinitychromatography.
Specificity
Recognizeshumanmyeloperoxidase(MPO).RecognizesnativeMPOinWesternBlotsandtheheavychainfollowingboilingofthesample.RecognizesrecombinantMPOinWesternBlotsandweaklyinELISA.TheantibodymaybeofvalueinthestudyofmyeloidcellsandmyeloidleukemiasbyFlowCytometryfollowingcellpermeabilization.
References
1.Audrain,M.A.P.,etal.,Clin.Exp.Immunol.107:127-134(1997).品牌介绍
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