Usbio/169807 Pro QPCR SuperMix生物基因组学与贸易；试剂盒，ROX预混合/169807/400反应
商品编号：
169807
品牌：
USbiological
市场价：
¥11420.00
美元价：
4853.50
产品分类：
克隆试剂盒
公司分类：
Cloning_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Componentsinthiskitarepreparedwithpurechemicalsaccordingtoourproprietarytechnology.ProQPCRSuperMix–ROXpremixedisa2xconcentrationofqPCRreagentincludingHotstartDNApolymeraseandaspeciallyformulatedrealtimebufferdesignedforreal-timePCRwithfluorescentprobe-baseddetectionformat.
Real–timeorquantitativePCR(QPCR)allowsquantificationofDNA,CDNA,orRNAtemplates.QPCRisbasedonthedetectionofafluorescentreportermoleculethatincreasesasPCRproductsaccumulatewitheachcycleofamplification.Theuseoffluorescentprobetechnologiesreducestheriskofnonspecificproductswhilemaintainingconvenience,speedandhighthroughputscreeningcapABIlities.TheProQPCRSuperMixisaready-to-use,2xconcentratedmastermixthatcontainsallthereagents(exceptprimers,probeandtemplates)neededforrunningquantitativereal-timeDNAdetectionassaysutilizingfluorescentprobe-basedtechnologiessuchasTaqMan®ormolecularbeacons.ThepassivereferencedyeROXispremixedintheProQPCRSuperMix–ROXpremixed.
QPCRSuperMixcontainsUnitedStatesBIOLOGical’sTaqDNApolymerasewithhotstartcapability.hot-startTaqDNApolymeraseimprovesPCRamplificationreactionsbydecreasingnonspecificamplificationandpreventingprimer-dimerformation.Thisenzymeisactivatedafteraninitialseventotenminutesheatingstepat95°C.Thereal-timePCRbufferisspeciallyformulatedtoprovidesuperiorspecificityandincreaseamplificationefficiency.ThisSuperMixcanamplifyanddetectabroadrangeofDNAsorcDNAs,includingthoseGC-orAT-richtargets.
KitComponents
ProQPCRSuperMixROXreferenceDye
StorageandStability
Storepowderat4°Cliquidat-20°C.Storeothercomponentsat4°C.Stableforatleast6monthsFormaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
References
1.HiguchiR,DollingerG,WalshPSandGriffithR(1992):Simultaneousamplificationanddetection|ofspecificDNAsequences.BioTechnology10:413-417.2.HiguchiR,FocklerC,DollingerGandWatsonR(1993):KineticPCRanalysis:real-timemonitoringofDNAamplificationreactions.BioTechnology11:1026-10303.Bustin,SA(2000):AbsolutequantificationofmRNAusingreal-timereversetranscriptionpolymerasechainreactionassays.JournalofMolecularEndocrinology25:169-193.品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
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