Eachkitcontains20individual10-merprimers(suppliedataminimumamountof50nmoleperprimer).TheseprimersaresuitableforuseingeneticmappingandDNAfingerprinting.IntheRAPDtechnique,asingle10merofarbitrarysequenceisusedasaprimerinPCRtoamplifygenomicDNAwherethesequenceoftheDNAiscompletelyunknown.GenomicDNAfromdifferentindividualsgivesdifferentPCRproducts,allowingtheidentificationofDNApolymorphismsthatcanbeusedtoidentifydifferentindividualsorasgeneticMarkers.|ThenumberofPCRproductsgeneratedfromeachgenomicDNAsampledependsontheprimersequence,thegenomicDNAsequence,andthegenomesize.Assumingthattheprimingsitesofa10merRAPDprimerarerandomlydistributedthroughoutagenome,thetheoreticalnumberofPCRproductsisapproximately2.5x10e9xG,whereGisthesizeofthehaploidgenomeinbasepairs.Usingthiscalculation,ahaploidgenomeof2x10e9bp,forexample,isexpectedtogiveabout5PCRproducts.Thispredictionisincloseagreementwithexperimentalresults.
