Usbio/169806 Pro QPCR SuperMix生物基因组学与贸易；Kit/169806/200
商品编号：
169806
品牌：
USbiological
市场价：
¥9160.00
美元价：
3893.00
产品分类：
克隆试剂盒
公司分类：
Cloning_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Componentsinthiskitarepreparedwithpurechemicalsaccordingtoourproprietarytechnology.ProQPCRSuperMixisa2xconcentrationofpremixreagentincludingHotstartDNApolymeraseandspeciallyformulatedrealtimebufferdesignedforreal-timePCRwithfluorescentprobe-baseddetectionformat.
Real–timeorquantitativePCR(QPCR)allowsquantificationofDNA,CDNA,orRNAtemplates.QPCRisbasedonthedetectionofafluorescentreportermoleculethatincreasesasPCRproductsaccumulatewitheachcycleofamplification.Theuseoffluorescentprobetechnologiesreducestheriskofsamplecontaminationwhilemaintainingconvenience,speedandhighthroughputscreeningcapABIlities.ProQPCRSuperMixcanbeusedwithfluorescentprobetechnologiestoperformreal-timePCR.TheProQPCRSuperMixisaready-to-use,2x-concentratedmastermixthatcontainsallthereagents(exceptprimers,probeandtemplates)neededforrunningquantitative,real-timeDNAdetectionassays,inthefluorescentprobe-baseddetectionassayssuchasTaqMan®ormolecularbeacons.The|passivereferencedyeROXisincludedintheProQPCRSuperMixformanyreal-timeQPCRplatforms.
QPCRSuperMixcontainsUnitedStatesBIOLOGical’sTaqDNApolymerasewithhotstartcapability.hot-startTaqDNApolymeraseimprovesPCRamplificationreactionsbydecreasingnonspecificamplificationandpreventingprimer-dimerformation.Thisenzymeisactivatedafteraninitialseventotenminutesheatingat95°C.Andthereal-timePCRbufferisspeciallyformulatedtoprovidesuperiorspecificityandincreaseamplificationefficiency.ThisSuperMixcanamplifyanddetectabroadrangeofDNAsorcDNAs,includingthoseGC-orAT-richtargets.
KitComponents
ProQPCRSuperMixROXreferenceDye
StorageandStability
Storepowderat4°Cliquidat-20°C.Storeothercomponentsat4°C.Stableforatleast6monthsFormaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
References
1.HiguchiR,DollingerG,WalshPSandGriffithR(1992):Simultaneousamplificationanddetection|ofspecificDNAsequences.BioTechnology10:413-417.2.HiguchiR,FocklerC,DollingerGandWatsonR(1993):KineticPCRanalysis:real-timemonitoringofDNAamplificationreactions.BioTechnology11:1026-10303.Bustin,SA(2000):AbsolutequantificationofmRNAusingreal-timereversetranscriptionpolymerasechainreactionassays.JournalofMolecularEndocrinology25:169-193.品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
恭喜苏州蚂蚁淘获得usbio2020年授权书，成为usbio中国代理，购买usbio,请认准usbio代理-苏州蚂蚁淘！
联络我们
