Usbio/169767 Eva QPCR SuperMix BioGenomics™ Kit/169767/400Reactions
商品编号：
169767
品牌：
USbiological
市场价：
¥11840.00
美元价：
5032.00
产品分类：
克隆试剂盒
公司分类：
Cloning_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Componentsinthiskitarepreparedwithpurechemicalsaccordingtoourproprietarytechnology.QPCRSuperMixisa2xconcentrationofpremixreagentincludingHot-startDNApolymeraseandEvaDyeandspeciallyformulatedrealtimebufferdesignedforreal-timePCRwithintercalatorformat.
Real–timeorquantitativePCR(QPCR)allowsquantificationofDNA,CDNA,orRNAtemplates.|QPCRisbasedonthedetectionofafluorescentreportermoleculethatincreasesasPCR|productsaccumulatewitheachcycleofamplification.InEvaQPCRSuperMix,asuperiorgreenfluorescenceDNA-bindingdyeisusedforreal-timedetectionandquantitationofDNA.TheEvaQPCRSuperMixisaready-to-use,2x-concentratedmastermixthatcontainsallthereagents(exceptprimersandtemplates)neededforrunningquantitative,real-timeDNAdetectionassays,inthedouble-strandedDNAdyedetectionformat.ThepassivereferencedyeROXisincludedinaseparatetubetomaketheEvaSupermixadaptableformanyreal-timeQPCRplatforms.Apairofhumanbeta-actinprimersisalsoincludedinthekitasacontrol.
QPCRSuperMixcontainsTaqpolymerasewithhotstartcapABIlity.hot-startTaqpolymeraseimprovesPCRamplificationreactionsbydecreasingnonspecificamplificationandpreventingprimer-dimerformation.Thisenzymeisactivatedafteraninitialseventotenminutesheatingat95°C.Andthereal-timePCRbufferisspeciallyformulatedtoprovidesuperiorspecificityandincreaseamplificationefficiency.ThisSuperMixcanamplifyanddetectabroadrangeofDNAorcDNAtargets,includingthoseareGC-orAT-rich.
KitComponents
EvaQPCRSuperMixROXreferenceDyeHumanbeta-actincontrolF/Rprimerpair(25x)
StorageandStability
Storepowderat4°Cliquidat-20°C.Storeothercomponentsat4°C.Stableforatleast6monthsFormaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
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