IMBPplatesareideallysuitedforapplicationsrequiringthedetection,isolationand/orcharacterizationofrare,minoritycontaminatingsequences.Becauseamplificationwillamplifybothminorityandmajoritysequences,denaturingandannealingwillresultinvirtuallyalloftheminoritystrandspairingwithmajoritystrands,thusproducingmismatchcontainingDNA.TheseheteroduplexescanthenbeisolatedbytheIMBPmethod.ItispossIBLetoreleaseboundheteroduplexesfromIMBPandsubsequentlytoamplify,sequenceorclonethem.Forexample,wehavebeensuccessfulinenrichingforDNAfragmentsthatwereinitially1%orlessoftheoriginalDNAmix.Followingenrichment,theseminoritysequencesweredetectedasclearheterozygotesbybothmanualandautomatedsequencing.AswithallIMBPallications,goodresultsdependongoodinputDNA(misincorporationerrorsduringamplificationwilalsoproduceheteroduplexeswhichcanbeboundbyIMP/BPandincreasethebackground).||KitComponents:|*M9557-01B1:MicrotiterStrips,1x96wells|M9557-01B2:WashBuffer,10X,1x25ml|M9557-01B3:Streptavidin(HRP)(1mg/ml),1x10ul|M9557-01B4:Tetramethylbenzine(TMB),1x10ml|M9557-01B5:HeteroduplexControl(Biotin)(20ng/ul),1x10ul|M9557-01B6:HomoduplexControl(20ng/ul),1x10ul||StorageandStABIlity:|Store*M9557-01B1at-20°C.Storeothercomponentsat4°C.Stablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.||Notes:|ForKit,order1Kit|ForPlateonly,order1PlateImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBIOLOGical.

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