MultipleTagisaspeciallydesignedfusionproteinforuseasapositivecontrolforWesternblotorDotblot.Thefusionproteinisa40kDproteincontaining16commonlyusedtagsfromN-terminustoC-terminusintheorderasshowninthetable:||Tag Sequence|TrxMSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEY|QGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQL|KEFLDANLA|T7 CMASMTGGQQMG|HSV QPELAPEDPEDC|c-Myc EQKLISEEDLC|VSV-g YTDIEMNRLGK |Glu-Glu CEEEEYMPME|V5 GKPIPNPLLGLDST|E-tag GAPVPYPDPLEPR|Flag-tag DYKDDDDK|S-tag KETAAAKFERQHMDS|HA YPYDVPDYAC|KT3 KPPTPPPEPET|E2 GVSSTSSDFRDR|AU1 DTYRYI|AU5 TDFYLK|6xHis HHHHHH ||Applications:|SuitableforuseinWesternBlotandDotBlot.Otherapplicationsnottested.||RecommendedDilution:|WesternBlot:0.04-0.2ulperlaneofminigel.ForaccurateloADIng,dilutewithPBS.|Optimaldilutionstobedeterminedbytheresearcher.||StorageandStABIlity:|Storelyophilizedpowderat-20°Corlower.ReconstitutebyaddingsteriledH2O.Aliquotreconstitutedproducttoavoidrepeatedfreezingandthawing,andfreezeat-70°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.Source:E.coli|Purity:>95%bySDS-PAGEandCoomassieblutestaining.PurifiedfromE.colicelllysate.|Concentration:~1mg/ml(afterreconstitution)|Form:Suppliedasalyophilizedpowder.Reconstitutewith200ulofddH2O.||ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBIOLOGical.

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