SampleType:|Serum,plasma,tissuehomogenates,celllysates,cellculturesupernatesandotherBIOLOGicalfluids||IntendedUse:|ThekitisasandwichenzymeimmunoassayforinvitroquantitativemeasurementofIL6incaprineserum,plasma,tissuehomogenates,celllysates,cellculturesupernatesandotherbiologicalfluids.||Sensitivity:|0.138pg/ml||Range:|0.312-20pg/ml||Specificity:|ThisassayhashighsensitivityandexcellentspecificityfordetectionofHighSensitiveInterleukin6(IL6).Nosignificantcross-reactivityorinterferencebetweenHighSensitiveInterleukin6(IL6)andanalogueswasobserved.||Precision:|Intra-Assay:CV<10%|Inter-Assay:CV<12%||TestPrinciple:|Themicroplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoIL6.Standardsorsamplesarethenaddedtotheappropriatemicroplatewellswithabiotin-conjugatedantibodyspecifictoIL6.Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainIL6,biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacid|solutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofIL6inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandard|curve.||KitComponents:|96-wellstripplate,1xPlate|Standard,2xvial|DetectionReagentA,1×120ul|DetectionReagentB,1×120ul|TMBSubstrate,1×9ml|WashBuffer(30X),1×20ml|StandardDiluent,1×20ml|AssayDiluentA,1×12ml|AssayDiluentB,1×12ml|StopSolution,1×6ml||StorageandStABIlity:|Storeallcomponentsat4°C.Stablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBiological.

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