SampleType:|Serum,plasma,tissuehomogenates,celllysates,cellculturesupernatesandotherBIOLOGicalfluids||IntendedUse:|ThekitisasandwichenzymeimmunoassayforinvitroquantitativemeasurementofIFNaingallusserum,plasma,|tissuehomogenates,celllysates,cellculturesupernatesandotherbiologicalfluids.||Sensitivity:|5.4pg/ml||Range:|15.6-1000pg/ml||Specificity:|ThisassayhashighsensitivityandexcellentspecificityfordetectionofInstantInterferonAlpha(IFNa).Nosignificantcross-reactivityorinterferencebetweenInstantInterferonAlpha(IFNa)andanalogueswasobserved.||Precision:|Intra-Assay:CV<10%|Inter-Assay:CV<12%||TestPrinciple:|Themicroplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoIFNa.Standardsorsamplesarethenaddedtotheappropriatemicroplatewellswithabiotin-conjugatedantibodyspecifictoIFNa.Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainIFNa,biotin-conjugatedantibodyand|enzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofIFNainthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.||KitComponents:|96-wellstripplate,1xPlate|Standard,5xvial|DetectionReagentA,1×120ul|ReagentDiluent,1×300ul|TMBSubstrate,1×9ml|WashBuffer(30X),1×20ml|StandardDiluent,1×20ml|AssayDiluentA,1×12ml|StopSolution,1×6ml||StorageandStABIlity:|Storeallcomponentsat4°C.Stablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBiological.

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