SampleType:|Serum,plasmaandotherBIOLOGicalfluids||IntendedUse:|ThekitisasandwichenzymeimmunoassayforinvitroquantitativemeasurementofC1INHinratserum,plasmaandotherbiologicalfluids.||Sensitivity:|2.61ng/ml||Range:|6.25-400ng/ml||Specificity:|ThisassayhashighsensitivityandexcellentspecificityfordetectionofComplement1Inhibitor(C1INH).Nosignificantcross-reactivityorinterferencebetweenComplement1Inhibitor(C1INH)andanalogueswasobserved.||Precision:|Intra-Assay:CV<10%|Inter-Assay:CV<12%||TestPrinciple:|Themicroplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoC1INH.Standardsorsamplesarethenaddedtotheappropriatemicroplatewellswithabiotin-conjugatedantibodyspecifictoC1INH.Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainC1INH,biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofC1INHinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.||KitComponents:|96-wellstripplate,1xPlate|Standard,2xvial|DetectionReagentA,1×120ul|DetectionReagentB,1×120ul|TMBSubstrate,1×9ml|WashBuffer(30X),1×20ml|StandardDiluent,1×20ml|AssayDiluentA,1×12ml|AssayDiluentB,1×12ml|StopSolution,1×6ml||StorageandStABIlity:|Storeallcomponentsat4°C.Stablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBiological.

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