TheFibrinogen(FG)BioAssay™ELISAKit(Bovine)isaquantitativesandwichassayforthedetectionofFGinserum,plasma,tissuehomogenatesandotherBIOLOGicalfluids.||Sensitivity:|0.469ng/ml||DetectionRange:|0.781-50ng/ml||Specificity:|ThisassayhashighsensitivityandexcellentspecificityfordetectionofbovineFibrinogen.Nosignificantcrossreactivityorinterferencebetweenandanalogueswasobserved.||Precision:|Intra-Assay:CV<8%|Inter-Assay:CV<10%||TestPrinciple:|ThisELISAkitemploysthesandwichenzyme-linkedimmune-sorbentassaytechnology.Antiantibodywaspre-coatedonto96-wellplates.Thebiotinconjugatedanti-antibodywasusedasdetectionantibodies.Thestandards,testsamplesandbiotinconjugateddetectionantibodywereaddedtothewellssubsequently,andwashwithwashbuffer.HRP-Streptavidinwasaddedandunboundconjugateswerewashedawaywithwashbuffer.TMBsubstrateswereusedtovisualizeHRPenzymaticreaction.TMBwascatalyzedbyHRPtoproduceabluecolorproductthatchangedintoyellowafteraddingacidicstopsolution.Thedensityofyellowisproportionaltotheamountofsamplecapturedinplate.ReadtheO.D.absorbanceat450nminamicroplatereader,andthentheconcentrationofcanbecalculated.||KitComponents:|MicroELISAPlate(Dismountable),8×12|LyophilizedStandard,2xvial|Sample/Standarddilutionbuffer,1x10ml|Biotin-detectionantibody(Concentrated),1x60ul|Antibodydilutionbuffer,1x5ml|HRP-StreptavidinConjugate(SABC),1x60ul|SABCdilutionbuffer,1x5ml|TMBsubstrate,1x5ml|Stopsolution,1x5ml|Washbuffer(25X),1x15ml|PlateSealer,1x5pieces||StorageandStABIlity:|Storeunopenedkitat4ºC;storeat-20ºConceopened.Storeunopenedstandardat4°C;oncereconstituted,storeat4°Cforupto12hoursorat-20°Cforupto48hours.Storeothercomponentsat4°C.Kitisstablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialsafterthawingandpriortoremovingthecap.ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBiological.ToxicityandHazards: Allproductsshouldbehandledbyqualifiedpersonnelonly,trainedinlaboratoryprocedures.

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