SampleType:|Tissuehomogenates,celllysatesandotherBIOLOGicalfluids||IntendedUse:|ThekitisasandwichenzymeimmunoassayforinvitroquantitativemeasurementofCASP3inrabbittissuehomogenates,celllysatesandotherbiologicalfluids.||Sensitivity:|5.7pg/ml||Range:|15.6-1000pg/ml||Specificity:|ThisassayhashighsensitivityandexcellentspecificityfordetectionofCaspase3(CASP3).Nosignificantcross-reactivityorinterferencebetweenCaspase3(CASP3)andanalogueswasobserved.||Precision:|Intra-Assay:CV<10%|Inter-Assay:CV<12%||TestPrinciple:|Themicroplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoCASP3.Standardsorsamplesarethenaddedtotheappropriatemicroplatewellswithabiotin-conjugatedantibodyspecifictoCASP3.Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainCASP3,biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofCASP3inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.||KitComponents:|96-wellstripplate,1xPlate|Standard,2xvial|DetectionReagentA,1×120ul|DetectionReagentB,1×120ul|TMBSubstrate,1×9ml|WashBuffer(30X),1×20ml|StandardDiluent,1×20ml|AssayDiluentA,1×12ml|AssayDiluentB,1×12ml|StopSolution,1x6ml||StorageandStABIlity:|Storeallcomponentsat4°C.Stablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBiological.

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