EIF5A2,amemberofeukaryoticinitiationfactor5Asubfamily,isanessentialproteintightlylinkedtocellularpolyaminehomeostasis.ThepreciseroleofeIF-5Ainproteinbiosynthesisisnotknownbutitfunctionsbypromotingtheformationofthefirstpeptidebondduringtheinitialstageofproteinsynthesis.Itseemstobetheonlyeukaryoticproteintohaveahypusineresidue,whichisapost-translationalmodificationofalysinebytheadditionofabutylaminogroup.||Source:|Recombinantcorrespondingtoa1-153aofhumanEIF5A26xhistaggedexpressedinE.coli.||AASequences:|MGSSHHHHHHSSGLVPRGSHMADEIDFTTGDAGASSTYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDYQLICIQDGYLSLTETGEVREDLKLPEGELGKEIEGKYNAGEDVQVSVMCAMSEEYAVAIKPCK||MolecularWeight:18.9kD(173aa),confirmedbyMALDI-TOF||StorageandStABIlity:|Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing..Storeat-20°C.Aliquotsarestableforatleast6monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.Source:E.coli|Purity:~85%(SDS-PAGE).|Concentration:~0.5mg/ml|Form:Suppliedasaliquidin20mMTris-HCl,pH8.0,10%glycerol.||ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBIOLOGical.

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