Source:|RecombinantcorrespondingtoCD33signalpeptide(aa1-16)andhumancathepsinS(aa17-331)withC-terminalHIStagexpressedinFreeStyle293-Fcells(NM_004079.3).||SpecificActivity:|8100pmol/min/ng.AssayCondition:50mMsodiumacetate,pH4.5,0.25Msodiumchloride,5mMDTT,10uMsubstrate(Mca-R-P-K-P-V-E-Nval-W-R-K(Dnp)-NH2),10minutesatRT.Enzymewaspreactivatedin50mMsodiumacetate,pH4.5,0.25Msodiumchloride,5mMDTTbufferfor2hoursatRTpriortoreaction.||Applications:|Suitableforuseinthestudyofenzymekinetics,screeninginhibitorsandselectivityprofiling.Otherapplicationsnottested.||RecommendedDilution:|Optimaldilutionstobedeterminedbytheresearcher.||StorageandStABIlity:|Aliquottoavoidrepeatedfreezingandthawing.Storeat-70°C.Aliquotsarestableforatleast6monthsat-70°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.Source:FreeStyle293-Fcell|Purity:Purified≥90%|Form:Suppliedasaliquidin25mMTris-HCl,pH8.0,100mMsodiumchloride,0.05%tween-20,10%glycerol.||ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBIOLOGical.

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