SpralsoknownasSepiapterinreductase,belongstotheShort-chaindehydrogenase/reductase(Sdr)familyandalsoreducesvariousexogenouscarbonylcompoundsincludingphenylpropanedione.Sprisanessentialenzymeforthebiosynthesisoftetrahydrobiopterin,anessentialcofactorforaromaticaminoacidhydrolasesincludingtyrosinehydroxylase,therate-limitingenzymeindopaminesynthesis.DefectsinSprcauseDOPA-responsivedystoniadefinedbythepresenceofsustainedinvoluntarymusclecontractions,oftenleADIngtoabnormalpostures.||Source:|Recombinantproteincorrespondingtoaa1-262frommouseSpr,fusedtoHis-tagatN-terminal,expressedinE.coli.||MolecularWeight:|~30.3kD(285aa),confirmedbyMALDI-TOF||AASequence:|MGSSHHHHHHSSGLVPRGSHMGSMEAGGLGCAVCVLTGASRGFGRALAPQLARLLSPGSVMLVSARSESMLRQLKEELGAQQPDLKVVLAAADLGTEAGVQRLLSAVRELPRPEGLQRLLLINNAATLGDVSKGFLNVNDLAEVNNYWALNLTSMLCLTSGTLNAFQDSPGLSKTVVNISSLCALQPYKGWGLYCAGKAARDMLYQVLAAEEPSVRVLSYAPGPLDNDMQQLARETSKDPELRSKLQKLKSDGALVDCGTSAQKLLGLLQKDTFQSGAHVDFYDC||StorageandStABIlity:|Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Source:Recombinant,E.coli|Purity:~95%(SDS-PAGE)|Concentration:~1mg/ml|Form:Suppliedasaliquidin20mMTris-HCl,pH8.5,1mMDTT,10%glycerol.||ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBIOLOGical.

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