RNA-directedRNApolymerasethatcatalyzesthetranscriptionofviralmRNAs,theircappingandpolyadenylation.ThetemplateiscomposedoftheviralRNAtightlyencapsidatedbythenucleoprotein(N).TheviralpolymerasebindstothegenomicRNAatthe3'leaderpromoter,andtranscribessubsequentlyallviralmRNAswithadecreasingefficiency.Thefirstgeneisthemosttranscribed,andthelasttheleasttranscribed.Theviralphosphoproteinactsasaprocessivityfactor.CappingisconcommitantwithinitiationofmRNAtranscription.Indeed,aGDPpolyribonucleotidyltransferase(PRNTase)addsthecapstructurewhenthenascentRNAchainlengthhasreachedfewnucleotides.Ribose2'-OmethylationofviralmRNAcapprecedesandfacilitatessubsequentguanine-N-7methylation,bothactivitiesbeingcarriedbytheviralpolymerase.PolyadenylationofmRNAsoccurbyastutteringmechanismatasliperystopsitepresentattheendviralgenes.AfterfinishingtranscriptionofamRNA,thepolymerasecanresumetranscriptionofthedownstreamgene.RNA-directedRNApolymerasethatcatalyzesthereplicationofviralgenomicRNA.ThetemplateiscomposedoftheviralRNAtightlyencapsidatedbythenucleoprotein(N).ThereplicasemodeisdependentonintracellularNproteinconcentration.Inthismode,thepolymerasereplicatesthewholeviralgenomewithoutrecognizingtranscriptionalsignals,andthereplicatedgenomeisnotcapedorpoly.||Source:|Partialrecombinantproteincorrespondingtoaa625-809ofZaireEbolavirus(strainMayinga-76)RNA-DirectedRNAPolymeraseL,fusedto6xHis-TagatN-terminal,expressedinE.coli.||MolecularWeight:|~24.9kD||AASequence:|RGSSFVTDLEKYNLAFRYEFTAPFIEYCNRCYGVKNVFNWMHYTIPQCYMHVSDYYNPPHNLTLENRDNPPEGPSSYRGHMGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVMGDNQCITVLSVFPLETDADEQEQSAEDNAARVAASLAKVTSACGIFLKPDETFVHSGFIYFGKKQYLNG||StorageandStABIlity:|Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor6monthsafterreceiptat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.

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