Brucellosisisaninfectiousdiseasecausedbysmallellipsoid,gram-negativebacteria.Therearefourdifferentgerms:Br.abortus,Br.melitensis,Br.suisandBr.canis.Peopleareinfectedbycontactwithinfectedanimalsorbyeatingmeatordrinkingunpasteurizedmilkfrominfectedanimals.Asarule,infectedhumansarenotcontagious.Brucellosisismostfrequentinyoungandmiddle-agedindividuals.Endangeredpersonsarebutchers,farmers,ownersofpets,veterinariesandtouristsinSoutherncountries.Theappearanceofbrucellosisshowsaprevalenceduringwinterandspring.|Theincubationperiodisbetweenoneandthreeweeks,butmaybeaslongastwomonths.Br.abortusandBr.melitensismaycauseBang’sDisease,orinrarecasesMaltaFever.Thefirstappearsoccasionallywithalowpathogenicityforman.TypicalsymptomsforBang’sDiseaseareperiodicallyoccurringfever,splenomegalyandswellingoflymphnodes.Insomecasesaninflammationofdifferentjointsandorgansoccurs.TheMaltaFeveriscausedbytheepidemictypeofbrucellosis,andinfectionalmostalwaysleadstoamanifestillness.SomeinfectionswithBrucellacancauseBrucellaHepatitis.ItispossIBLethatthereisalinkbetweenaninfectionwithBrucellaandtheoutbreakofmultiplesclerosis.|Duringanantibiotictherapyorachronicinfection,thedetectionofBrucellaspec.inblood,urine,cerebrospinalfluid,sputumorotherbodyfluidscouldbenegative.SEROlogicalmethodslikeagglutination,complementfixationreaction,BrucellaCoombstestandELISAaregoodalternatives.Themonitoringofantibodiescanserveasausualindicationofthestatusofinfection.Duringthefirstdays,IgMistheonlyimmunoglobulinthatappears.Asthediseaseprogresses,IgMrecedesquantitativelyandIgGbecomespredominant.Inchronicbrucellosis,IgGmaybeproducedforanextendedperiod.||IntendedUse:|TheBrucellaIgMAntibodyELISATestKithasbeendesignedforthethedetectionandthequantitativedeterminationofspecificIgMantibodiesagainstBrucellainserumandplasma.FurtherapplicationsinotherbodyfluidsarepossibleandcanberequestedfromtheTechnicalServiceofUnitedStatesBIOLOGical.Thisassayisintendedforin-vitrodiagnosticuseonly.|Laboratoryresultscanneverbetheonlybaseofamedicalreport.Thepatienthistoryandfurthertestshaveadditionallytobetakenintoaccount.||PrincipleoftheTest:|TheBrucellaIgMantibodytestkitisbasedontheprincipleoftheenzymeimmunoassay(EIA).Brucellaantigenisboundonthesurfaceofthemicrotiterstrips.Dilutedpatientserumorready-to-usestandardsarePipettedintothewellsofthemicrotiterplate.AbindingbetweentheIgMantibodiesoftheserumandtheimmobilizedBrucellaantigentakesplace.Afteraonehourincubationatroomtemperature,theplateisrinsedwithdilutedwashsolution,inordertoremoveunboundmaterial.Thenready-to-useanti-human-IgMperoxidaseconjugateisaddedandincubatedfor30minutes.Afterafurtherwashingstep,thesubstrate(TMB)solutionispipettedandincubatedfor20minutes,inducingthedevelopmentofabluedyeinthewells.Thecolordevelopmentisterminatedbytheadditionofastopsolution,whichchangesthecolorfrombluetoyellow.Theresultingdyeismeasuredspectrophotometricallyatthewavelengthof450nm.TheconcentrationoftheIgMantibodiesisdirectlyproportionaltotheintensityofthecolor.||Specificity:100%||Sensitivity:100%||KitComponents:|167545A:Microtiterplate,1x12Brucellaantigencoatedmicrotiterstrip|167545B:CalibratorA(NegativeControl),1x2ml|167545C:CalibratorB(Cut-OffStandard),1x2ml|167545D:CalibratorC(WeakPositiveControl),1x2ml|167545E:CalibratorD(PositiveControl),1x2ml|167545F:EnzymeConjugate,15ml|167545G:Substrate,1x15ml|167545H:StopSolution,1x15ml|167545K:SampleDiluent,1x60ml|167545L:WashSolution10X(PBS+Tween20),1x60ml|WashBuffer(10×),1x60ml||StorageandStABIlity:|Storeallcomponentsat4°C.Stablefor6months.Reconstitutedstandardsarestablefortwoweeksstoredat4ºC.Formaximumrecoveryofproduct,centrifugetheoriginalvialpriortoremovingthecap.

usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。 恭喜苏州蚂蚁淘获得usbio2020年授权书，成为usbio中国代理，购买usbio,请认准usbio代理-苏州蚂蚁淘！