Gliadin IgA ELISA test system is designed to detect IgA class antibodies to Gliadin in human sera. Wells of plastic microwell strips are sensitized by passive absorption with Gliadin antigen. The test procedure involves three incubation steps:|1. Test sera (properly diluted) are incubated in antigen coated microwells. Any antigen specific antibody in the sample will bind to the immobilized antigen. The plate is washed to remove unbound antibody and other serum components.|2. Peroxidase Conjugated goat anti-human IgA is added to the wells and the plate is incubated. The Conjugate will react with Gliadin antibody immobilized on the solid phase in step 1. The wells are washed to remove unreacted Conjugate.|3. The microwells containing immobilized peroxidase Conjugate are incubated with peroxidase Substrate Solution. Hydrolysis of the Substrate by peroxidase produces a color change. After a period of time the reaction is stopped and the color intensity of the solution is measured photometrically. The color intensity of the solution depends upon the antibody concentration in the original test sample.||Specificity:|96%||Sensitivity: |93.90%||KitComponents:|Microtiter plate, 1x96 wells |Conjugate (HRP) Reagent, 1x15ml|Positive Control (Human Serum), 1x350ul|Calibrator (Human Serum), 1x500ul|Negative Control (Human Serum), 1x350ul|Sample Diluent, 1x30ml|TMB Substrate, 1x15ml |Stop solution, 1x15ml|Wash Buffer (10X), 1x100ml||Storage and Stability:|Store at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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