Anti-tetanus toxoid antibodies are raised in response to vaccination with tetanus toxoid protein. A patient’s response to the immunization may be assessed, subsequently, by the serological determination of their anti-tetanus toxoid antibody levels using this quantitative enzyme immunoassay technique. Patients with recurrent infections should be investigated for immunodeficiency due to thymic abnormalities, and the consequent inability to respond to specific bacterial antigens (review1). ||Intended Use:|Anti-Tetanus is intended for the in-vitro measurement of specific IgG antibodies against tetanus toxoid (T.Tox.) present in serum, in order to determine protective status. Sufficient materials are supplied to allow a maximum of 39 samples to be tested in duplicate or 87 in single, with a seven point calibration curve and two controls. The seven point calibration curve can be reduced to five points if measurement below 0.09 IU/ml is not required.||Sensitivity: 0.0093 IU/ml||Range: 0.01-7.0IU/ml||Specificity: 97.10%||Test Principle:|Microwells are pre-coated with tetanus toxoid antigen. The calibrators, controls and diluted patient samples are added to the wells and antibodies recognising the tetanus toxoid antigen bind during the first incubation. After washing the wells to remove all unbound proteins, purified peroxidase labelled rabbit anti- human IgG (y chain specific) conjugate is added. The conjugate binds to the captured human antibody and the excess unbound conjugate is removed by a further wash step. The bound conjugate is visualised with 3,3’,5,5’ tetramethylbenzidine (TMB) substrate which gives a blue reaction product, the intensity of which is proportional to the concentration of antibody in the sample. Phosphoric acid is added to each well to stop the reaction. This produces a yellow end point color, which is read at 450nm. ||KitComponents:|1. Coated Wells, 12x8well strips|2. Sample Diluent, 2x50ml|3. IgG Calibrators, 7x1.2ml|4. IgG High Control, 1x1.2ml|5. IgG Low Control, 1x1.2ml|6. IgG Conjugate, 1x12ml|7. TMB Substrate, 1x14ml|8. Stop Solution, 1x14ml||Storage and Stability:|Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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