TheCNKSR1(ConnectorEnhancerofKinaseSuppressorofRas1)BioAssay™ELISAKit(Human)isaquantitativesandwichassayforthedetectionofCNKSR1inhumanserum,plasmaandotherBIOLOGicalfluids.Celllysatesandtissuehomogenates,thoughnottested,maypotentiallybeusedassamples.||Sensitivity:|0.188ng/ml||DetectionRange:|0.313-20ng/ml||Specificity:|ThisassayhashighsensitivityandexcellentspecificityfordetectionofhumanCNKSR1.Nosignificantcross-reactivityorinterferencebetweenCNKSR1andanalogueswasobserved.||Precision:|Intra-Assay:CV<8%|Inter-Assay:CV<10%||TestPrinciple:|ThisELISAkitemploysthesandwichenzyme-linkedimmune-sorbentassaytechnology.Anti-CNKSR1antibodywaspre-coatedonto96-wellplates.Thebiotinconjugatedanti-CNKSR1antibodywasusedasdetectionantibodies.Thestandards,testsamplesandbiotinconjugateddetectionantibodywereaddedtothewellssubsequently,andwashedwithwashbuffer.HRP-Streptavidinwasaddedandunboundconjugateswerewashedawaywithwashbuffer.TMBsubstrateswereusedtovisualizeHRPenzymaticreaction.TMBwascatalyzedbyHRPtoproduceabluecolorproductthatchangedintoyellowafteraddingacidicstopsolution.ThedensityofyellowisproportionaltotheCNKSR1amountofsamplecapturedinplate.ReadtheO.D.absorbanceat450nminamicroplatereader,andthentheconcentrationofCNKSR1canbecalculated.||KitComponents:|MicroELISAPlate(Dismountable),8×12|LyophilizedStandard,2xvial|Sample/Standarddilutionbuffer,1x20ml|Biotin-labeledantibody(Concentrated),1x120ul|Antibodydilutionbuffer,1x10ml|HRP-StreptavidinConjugate(SABC),1x120ul|SABCdilutionbuffer,1x10ml|TMBsubstrate,1x10ml|Stopsolution,1x10ml|Washbuffer(25X),1x30ml|PlateSealer,1x5pieces||StorageandStABIlity:|Storeunopenedkitat4ºC;storeat-20ºConceopened.Storeunopenedstandardat4°C;oncereconstituted,storeat4°Cforupto12hoursorat-20°Cforupto48hours.Storeothercomponentsat4°C.Kitisstablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialsafterthawingandpriortoremovingthecap.

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