The Beta-EP (Beta-Endorphin) BioAssay™ ELISA Kit (Sheep) is a quantitative competitive assay for the detection of beta-EP in serum, plasma, tissue homogenates and other biological fluids||Sensitivity:|9.375pg/ml||Detection Range:|15.625-1000pg/ml||Specificity:|This assay has high sensitivity and excellent specificity for detection of beta-EP. No significant cross-reactivity or interference between beta-EP and analogues is observed.||Precision:|Intra-Assay: CV<8%|Inter-Assay: CV<10%||Test Principle:|The microtiter plate provided in this kit has been pre-coated with beta-EP. During the reaction, beta-EP in the sample or standard competes with a fixed amount of beta-EP on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to beta-EP. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate|reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of beta-EP in the samples is then determined by comparing the O.D. of the samples to the standard curve.||Kit Components:|Micro ELISA Plate(Dismountable), 8×12|Lyophilized Standard, 2x vial|Sample / Standard dilution buffer, 1x20ml|Biotin-detection antibody (Concentrated), 1x60ul|Antibody dilution buffer, 1x10ml|HRP-Streptavidin Conjugate(SABC), 1x120ul|SABC dilution buffer, 1x10ml|TMB substrate, 1x10ml|Stop solution, 1x10ml|Wash buffer (25X), 1x30ml|Plate Sealer, 1x5pieces||Storage and Stability:|Store unopened kit at 4ºC; store at -20ºC once opened. Store unopened standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

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