For quantitative detection of RF-IgM in serum, plasma, tissue homogenates and other biological fluids.||Sensitivity:|2.813IU/ml||Detection Range:|4.69-300IU/ml||Specificity:|This assay has high sensitivity and excellent specificity for detection of mouse RF-IgM. No significant cross-reactivity or interference between RF-IgM and analogues was observed.||Precision:|Intra-Assay: CV<8%|Inter-Assay: CV<10%||Test Principle:|This ELISA kit employs the indirect enzyme-linked immune-sorbent assay technology. Mouse IgG was pre-coated onto 96-well plates. The biotin conjugated anti- RF-IgM antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RF-IgM amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RF-IgM can be calculated.||Kit Components:|Micro ELISA Plate(Dismountable), 8×12|Lyophilized Standard, 2x vial|Sample / Standard dilution buffer, 1x20ml|Biotin-labeled antibody (Concentrated), 1x120ul|Antibody dilution buffer, 1x10ml|HRP-Streptavidin Conjugate(SABC), 1x120ul|SABC dilution buffer, 1x10ml|TMB substrate, 1x10ml|Stop solution, 1x10ml|Wash buffer (25X), 1x30ml|Plate Sealer, 1x5pieces||Storage and Stability:|Store unopened kit at 4ºC; store at -20ºC once opened. Store unopened standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

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