Cpf1-containing CRISPR-Cas loci of Francisella tularensis subsp. novicida U112 encode functional defense systems capable of mediating plasmid interference in bacterial cells guided by the CRISPR spacers. Unlike Cas9 systems, Cpf1-containing CRISPR systems have three features: First, Cpf1-associated CRISPR arrays are processed into mature crRNAs without the requirement of an additional trans-activating crRNA (tracrRNA). Second, Cpf1-crRNA complexes efficiently cleave target DNA proceeded by a short T-rich protospacer adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9 systems. Third, Cpf1 introduces a staggered DNA double stranded break with a 4 or 5-nt 5’ overhang.||Applications:|Suitable for use in Western Blot, ELISA and Immunofluorescence. Other applications not tested.||Recommended Dilution:|Western Blot: 1ug/ml|ELISA: 0.005-1ug/ml|Immunofluorescence: 0.2-1ug/ml|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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