TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant rat TPO is a fully biologically active 174aa polypeptide (18.7kD), which contains the erythropoietin-like domain of the full length TPO protein.||Source:|Recombinant protein corresponding to rat Thrombopoeitin, expressed in E.coli.||Biological Activity:|Determined by the dose-dependent stimulation of the proliferation of human MO7e cells. The ED50 was found to be 0.2ng/ml||Specific Activity:|5x10e7units/mg||Endotoxin:|<0.1ng/ug (1EU/ug)||AA Sequence:|SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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