Transforming Growth Factor-alpha (TGF-a) stimulates the proliferation of a wide range of epidermal and epithelial cells. TGF-a has been shown to play a role in the autocrine growth of certain transformed cells. ||Source:|Recombinant protein corresponding to 50aa residues from human Transforming Growth Factor-alpha, expressed in E. coli. ||Molecular Weight: |~5.5kD||Biological Activity:|The ED50, as determined by the dose-dependent stimulation of proliferation of Balb-c 3T3 cells, is <0.2ng/ml, corresponding to a specific activity of >5x10e6units/mg. For most in vitro applications, TGF-a exerts its biological activity in the concentration range of 0.5-25ng/ml.||Endotoxin:|<0.1ng/ug||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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