C-QNQRMKSKRWQKNN,fromtheinternalregionofhumanNANOG.|Controlpeptidecorrespondingtopolyclonalantibody,Catalog#N0019-01K,goatxhuman.||NANOGisauniquevariantofNK2familyoftranscriptionfactors.ItisahumanorthologofmouseNanogandconsistsofahighlyconservedhomeodomainandTrp-richrepeats.Itisspecificallyexpressedbyembryonicstemcellsandiscrucialforsecondembryoniclineagedetermination.ItworksinconcertwithOCT-4andSOX-2andplaysacentralroleinregulatingpluripotencyandself-renewalofICMandEScells.ItfunctionsindependentofLIF/gp130/Stat3pathwayandpreventsdifferentiationofEScellstoextraembryonicendodermandtrophectodermlineages.Itsexpressionhasalsobeendetectedinembryoniccarcinomacellsandgermlinetumorsindicatingatherapeuticintervention||Applications:|SuitableforuseinELISA.OtherApplicationsnottested.||RecommendedDilutions:|Optimaldilutionstobedeterminedbytheresearcher||StorageandStABIlity:|Maybestoredat4°Cforshort-termonly.Forlong-termstorageandtoavoidrepeatedfreezingandthawing,aliquotandstoreat-20°C.Aliquotsarestableforatleast12monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.||PeptideBlocking(seecorrespondingantibodyN0019-01K):|Antibodiesaretypicallysuppliedat0.5mg/mlandpeptidesasa100ulpellet.Whenpeptidesarereconstitutedin200ulwater,theconcentrationwouldbealso0.5mg/ml.Tostart,thebestratiowouldbe1:1(whichmeansmolarexcessofpeptidesrelativetoantibodieswhenidenticalvolumesaremixed).Mixequalvolumesofpeptideandantibodyattherequireddilutionandleaveatambienttemperature.Itisbestistohavetwoidenticalblots,tobeincubatedwithequalamountofantibodies,butonewiththeantibodiespre-adsorbedtothepeptidefor20min.Thenincubateanddevelopthetwoblotsinparallel.

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