SQRPSRCSNPTPGP,CTerminusofhumanFOXP3.|Controlpeptidecorrespondingtopolyclonalantibody,Catalog#F9050-10A,goatxhuman.||FOXP3(a431aminoacidprotein)isamemberoftheforkhead/winged-helixfamilyoftranscriptionalregulatorsandishighlyconservedacrossmammals.FOXP3isessentialfornormalimmunehomeostasis.||FOXP3isstablyandconstitutivelyexpressedatahighlevelinCD25+CD4positiveregulatoryTcells,atlowlevelinCD4positive/CD25negativecells,andisabsentinCD4negative/CD8positiveTcells.FOXP3maybeamasterregulatorygeneandamorespecificMarkerofregulatoryTcellsthanotherTcells.||Applications:|SuitableforuseinELISA.Otherapplicationsnottested.||RecommendedDilution:|Optimaldilutiontobedeterminedbyresearcher.||StorageandStABIlity:|Maybestoredat4°Cforshort-termonly.Forlong-termstorageandtoavoidrepeatedfreezingandthawing,add40-50%glycerol,aliquotandstoreat-20°C.Aliquotsarestableforatleast12monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuf||PeptideBlocking(seecorrespondingantibodyF9050-10A):|Antibodiesaretypicallysuppliedat0.5mg/mlandpeptidesasa100ulpellet.Whenpeptidesarereconstitutedin200ulwater,theconcentrationwouldbealso0.5mg/ml.Tostart,thebestratiowouldbe1:1(whichmeansmolarexcessofpeptidesrelativetoantibodieswhenidenticalvolumesaremixed).Mixequalvolumesofpeptideandantibodyattherequireddilutionandleaveatambienttemperature.Itisbestistohavetwoidenticalblots,tobeincubatedwithequalamountofantibodies,butonewiththeantibodiespre-adsorbedtothepeptidefor20min.Thenincubateanddevelopthetwoblotsinparallel.

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