Source: |Recombinant protein corresponding to 227aa human Oncostatin M expressed in E. coli.||Molecular Weight: |~26kD||Endotoxin:|~0.1ng/ug||Biological Activity: |ED50 <2ng/ml ( > 5x105units/mg). The biological activity is determined by measuring the oncostatin M concentration dependent stimulation of proliferation of TF-1 cells. The suggested concentration range for in vitro activity studies is 0.2-20.0ng/ml. The optimal concentration should be determined for each specific application. Oncostatin M is observed to exert variable, cell type dependent effects. For example, oncostatin M is observed to stimulate the growth of normal fibroblasts and AIDS-related Kaposi sarcoma cells, but is observed to inhibit the growth of A375 melanoma cells.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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