Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.\t||Source:|Beta Nerve Growth Factor, from human DNA sequence encoding the human beta NGF protein sequence (containing the signal peptide, pro-peptide and the mature beta NGF sequence) was expressed in modified human 293 cells.||Molecular Weight:|~12kD-16kD in SDS-PAGE and ~13.5kD molecular mass.||Biological Activity:|The ED50 of beta NGF is typically 0.2-1ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line.||AA Sequence:|YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEAR PVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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