The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. ||Source:|Recombinant protein corresponding to 115aa from human MIF, expressed in E. coli. ||Molecular Weight: |~12.5kD||Biological Activity:|Measured in an agarose micro droplet assay using human U937 cells as targets. The optimal range of activity found for human MIF in this assay was 0.5-1ug/ml.||Endotoxin:|<0.1ng/ug||Storage and Stability:|Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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