MCP-1 is an ~13.8kD peptide corresponding to full-length mature mouse monocyte chemotactic protein 1. MCP-1, otherwise known as CCL2 or JE, is a secreted chemokine involved in the recruitment of monocytes to sites of injury or inflammation (Lu, B. et al., 1998).||Source:|Recombinant protein corresponding to mouse MCP-1 prepared from E. coli.||Molecular Weight: |~13.8kD (125aa)||Applications: |Suitable for use in ELISA, Western Blot, Functional Assays. Other applications not tested.||Recommended Dilution:|ELISA: 0.2-0.4ng/well|Western Blot: 1.5-3ng/lane|Functional Assays: 1-20ng/ml|Optimal dilutions to be determined by the researcher.||Endotoxin Level: <0.1ng/ug||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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