Macrophage Colony stimulating Factor (M-CSF) is produced by various cell types, including fibroblasts, microphages, and endothelial cells. M-CSF is involved in both the development and function of monocytes/microphages. The recombinant M-CSF is a disulfide-linked homodimer composed of two 18.2kD subunits of 156aa residues.||Source:|Recombinant protein corresponding to mouse Macrophage Colony stimulating Factor, expressed in E. coli. ||Molecular Weight: |~36.4kD||Biological Activity:|The ED50, as determined by the dose-dependent stimulation of the proliferation of mouse M-NSF-60 cells, is <1.0ng/ml, corresponding to a specific activity of >1x10e6units/mg.||Endotoxin:|<0.1ng/ug||Storage and Stability:|Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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