Human resistin, a disulfide-linked homodimeric protein otherwise known as adipose tissue-specific secretory factor, is an adipose-derived hormone and member of the resistin/FIZZ family, implicated as a suppressor of the ability of insulin to reduce blood sugar, and to play a role in type 2 diabetes and obesity. Studies have shown that resistin is involved in an increase in the transcription/expression of many pro-inflammatory cytokines, including interleukin-1,-6 and -12, and also upregulates vascular cell-adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), and monocyte chemotactic protein-1 (MCP-1/CCL2).||Source:|Recombinant protein corresponding to human Resistin expressed in E. coli.||Molecular Weight: |~19.5kD||Biological Activity:|Determined by its ability to stimulate lipolysis in cultured human adipocytes.||Applications: |Suitable for use in ELISA, Western Blot and Functional Assays. Other applications not tested.||Recommended Dilution:|ELISA: 0.2-0.4ng/well|Western Blot: 1.5-3.0ng/lane|Optimal dilutions to be determined by the researcher.||Endotoxin: <1EU/ug||Storage and Stability:|Lyophilized powder may be stored at -20°C. Reconstitute with 250ul ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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