C-TRHEADSSPRGDPES,fromtheinternalregionofhumanHAX1.|Controlpeptidecorrespondingtopolyclonalantibody,Catalog#H7500-18,goatxhuman.||Theproteinencodedbythisgeneisknowntoassociatewithhematopoieticcell-specificLynsubstrate1,asubstrateofSrcfamilytyrosinekinases.Italsointeractswiththeproductofthepolycystickidneydisease2gene,mutationsinwhichareassociatedwithautosomal-dominantpolycystickidneydisease,andwiththeF-actin-bindingprotein,cortactin.Itwasearlierthoughtthatthisgeneproductismainlylocalizedinthemitochondria,however,recentstudiesindicateittobelocalizedinthecellbody.Mutationsinthisgeneresultinautosomalrecessiveseverecongenitalneutropenia,alsoknownasKostmanndisease.Twotranscriptvariantsencodingdifferentisoformshavebeenfoundforthisgene.||Applications:|SuitableforuseinELISA.Otherapplicationsnottested.||RecommendedDilutions:|Optimaldilutionstobedeterminedbytheresearcher.||StorageandStABIlity:|Maybestoredat4°Cforshort-termonly.Forlong-termstorageandtoavoidrepeatedfreezingandthawing,aliquotandstoreat-20°C.Aliquotsarestableforatleast12monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.||PeptideBlocking(seecorrespondingantibodyH7500-18):|Antibodiesaretypicallysuppliedat0.5mg/mlandpeptidesasa100ulpellet.Whenpeptidesarereconstitutedin200ulwater,theconcentrationwouldbealso0.5mg/ml.Tostart,thebestratiowouldbe1:1(whichmeansmolarexcessofpeptidesrelativetoantibodieswhenidenticalvolumesaremixed).Mixequalvolumesofpeptideandantibodyattherequireddilutionandleaveatambienttemperature.Itisbestistohavetwoidenticalblots,tobeincubatedwithequalamountofantibodies,butonewiththeantibodiespre-adsorbedtothepeptidefor20min.Thenincubateanddevelopthetwoblotsinparallel.

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