This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.||Recombinant full-length rat RSK1 (residues 1–end), containing additional N-terminal 6His and HA (YPYDVPDYA) tags, expressed in baculovirus in Sf21 insect cells||Specific Activity: |~4000-5000U/mg||Unit Definition:|One unit of RSK1 activity is defined as 1nmol phosphate incorporated into 30uM Rsktide/minute at 30°C with a final ATP concentration of 100uM.||Quality Control Testing:|Kinase Assay: 0.3–3.8ng phosphorylates 30uM RSKtide. Assay background was subtracted from the actual counts to yield the results shown below.|MS Tryptic Fingerprint: Confirmed product identity as rat RSK1 with the translated sequence. SDS-PAGE and Coomassie Stain: Purity was assessed by SDS-PAGE and Coomassie blue staining using 3ug of RSK1 active.||Storage and Stability:|Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Handling Recommendations: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

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