HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60kD alpha and 30kD beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human Pro-HGF shares 90%-93% aa sequence identity with bovine, canine, feline, mouse, and rat Pro-HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up-regulation of Integrin alpha2beta1 in epithelial cells by a selective increase in alpha2 gene transcription (11). This Integrin serves as a Collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of Nectin-1alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thymus, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).||Source:|Recombinant protein corresponding to aa32-728, Gln32-Ser728, from human HGF Propeptide, Spodoptera frugiperda, Sf 21 (baculovirus)-derived.||Molecular Weight: |~84kD||Endotoxin:|<0.10EU/1ug (LAL method).||Biological Activity:|>20ng of Recombinant Human HGF Propeptide is cleaved to release active HGF when treated for 4 hours at 37°C with 5ng of kallikrein.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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