Human granulocyte colony-stimulating factor(hG-CSF) is one of the hematopoietic growth factors which plays an important role in stimulating proliferation, differentiation, and functional activation of blood cells. In addition to its effect on hematopoiesis, G-CSF also enhances neutrophil functions against bacteria, fungi, and tumor cells mediated by antibody-dependent cell-mediated cytotoxicity. Recombinant hG-CSF was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.| |Molecular Weight: 18.8kD (175amino acids including N-terminal methionine)| |Sequence:|MTPLGPASSL/ PQSFLLKCLE/ QVRKIQGDGA/ ALQEKLCATY/ KLCHPEELVL/ LGHSLGIPWA/ PLSSCPSQAL/ QLAGCLSQLH/ SGLFLYQGLL/ QALEGISPEL/ GPTLDTLQLD/ VADFATTIWQ/ QMEELGMAPA/ LQPTQGAMPA/ FASAFQRRAG/ GVLVASHLQS/ FLEVSYRVLR/ HLAQP||Biological Activity:|Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is less than or equal to 0.3 ng/ml.||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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