ControlpeptideforN1193-16C.||KFQQREKKGKCKKAfromtheC-TerminusofhumanNetrin1.|Netrinisincludedinafamilyoflaminin-relatedsecretedproteins.Thefunctionofthisgenehasnotyetbeendefined;however,netrinisthoughttobeinvolvedinaxonguidanceandcellmigrationduringdevelopment.Mutationsandlossofexpressionofnetrinsuggestthatvariationinnetrinmaybeinvolvedincancerdevelopment.||Applications:|SuitableforuseinELISAandAntibodyBlockingOtherapplicationsnottested.||RecommendedDilution:|Optimaldilutionstobedeterminedbytheresearcher.||PositiveControl:|HumanBrain(Cerebellum),MouseandRatBrainlysates||StorageandStABIlity:|Lyophilizedpowdermaybestoredat-20°C.Stablefor12monthsat-20°C.ReconstitutewithsterilebufferorddH2O.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Reconstitutedproductisstablefor6monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.||PeptideBlocking:|Tostart,thebestratiowouldbe1:1(whichmeansmolarexcessofpeptidesrelativetoantibodieswhenidenticalvolumesaremixed).Mixequalvolumesofpeptideandantibodyattherequireddilutionandleaveatambienttemperature.Itisbestistohavetwoidenticalblots,tobeincubatedwithequalamountofantibodies,butonewiththeantibodiespre-adsorbedtothepeptidefor20min.Thenincubateanddevelopthetwoblotsinparallel.

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