FLT-3 ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. The human flt-3 ligand also stimulates the proliferation of cells expressing murine flt-3 receptors.n combination with SCF and IL-3 flt-3 ligand can cause expansion of cells with the marker spectrum CD34(+) CD38(-). Alone, flt-3 ligand supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU-GEMM, and the very primitive high proliferative potential colony-forming cells, HPP-CFC. Flt-3 ligand only has marginal effects on progenitors for erythroid cells and megakaryocytes.||Source:|Recombinant protein corresponding to mouse FLT3 Ligand expressed in E. coli.||Molecular Weight:|~19kD||Biological Activity:|The ED50 was determined by the dose-dependent proliferation of human AML5 cells was found to be in the range of 10ng/mL.||Endotoxin:|<1EU/ug (LAL)||Storage and Stability:|Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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