EPGN is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. EPGN is produced in numerous tissues, including the testis, liver, heart and in certain tumor cells. EPGN is mitogenic for fibroblasts and epithelial cells. Human EPGN is originally synthesized as a glycosylated 14.7kD transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence.||Source:|Recombinant protein corresponding to 72aa from human Epigen, expressed in E. coli. ||Molecular Weight: |~7.9kD||Biological Activity:|Determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells. The expected ED50 for this effect is 150-300ng/ml.||Endotoxin:|<0.1ng/ug||Storage and Stability:|Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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