Eotaxin-3 is a CC chemokine that signals through the CCR3 receptor. It is produced by endothelial cells stimulated with IL-4 or IL-13. Eotaxin-3 selectively targets cells expressing CCR3, including eosinophils, basophils, T cells and monocytes. Eotaxin-3 has similar activity to Eotaxin and Eotaxin-2, but the three Eotaxins share only a low degree of sequence homology. Recombinant human Eotaxin-3 is an 8.4kD protein containing 71aa residues, including the four highly conserved cysteine residues present in CC chemokines.||Source:|Recombinant protein corresponding to 71aa residues from human Eotaxin-3, expressed in E. coli. ||Molecular Weight: |~8.4kD||Biological Activity:|Determined by its ability to chemoattract human CCR3/HEK 293 cells using a concentration range of 1-2ug/ml.||Endotoxin:|<0.1ng/ug of protein (<1EU/ug)||AA Sequence:|TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L||Storage and Stability:|Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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