Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant human SCF is an 18.4kD polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF. Manufactured using all non-animal reagents.||Source:|Recombinant protein corresponding to human SCF, expressed in E. coli.||Appearance:|Lyophilized powder in 10mM Acetic Acid. Reconstitute with ddH2O.||Purity:|≥98% (SDS-PAGE, HPLC)||Biological Activity:|Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤2.0ng/ml, corresponding to a specific activity of ≥5x10e5units/mg.||Endotoxin:|≤0.1ng/ug of protein ||AA Sequence:|MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL|PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL|VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF|NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM|LPPVA||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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