Platelet factor 4 (PF4/CXCL4) is an ELR− tetrameric, cationic chemokine that constitutes 25% of the protein in platelet alpha-granules. It is also found bound to the luminal vascular endothelial surface. Although platelets represent the primary source of PF4, it is also expressed at lower levels in other cells of the immune system including cultured T cells, monocytes, and endothelial and smooth muscle cells.||Source:|Recombinant protein corresponding to mouse CXCL4 expressed in E. coli.||Molecular Weight:|~9kD||Biological Activity:|Determined by its ability to inhibit human FGF-basic-dependent proliferation of NR6R-3T3 mouse fibroblasts. The ED50 for this effect is typically 5-15ug/mL.||Endotoxin:|<1EU/ug (LAL)||Storage and Stability:|Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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