Chemokine (CXC motif) ligand 14, also known as breast and kidney-expressed chemokine (BRAK), is a CXC chemokine that is a potent chemoattractant for neutrophils but not for T-cells, B-cells, monocytes or granulocytes. Mature CXCL14 has many conserved features of the CXC family and is 30% homologous to the MIP-alpha and MIP-beta sequences, but differs in that it has an additional five amino acid sequence in between the third and fourth cysteines and a short N-terminal. It is usually highly expressed near its fibroblast source and in epidermal fibroblasts and keratinocytes of skin, but is absent from cancer cells.||Source:|Recombinant protein corresponding to human CXCL14 expressed in E. coli.||Molecular Weight:|~9.6kD||Biological Activity:|The ED50 is 0.25-1.25ng/mL as determined by its ability to induce calcium flux in activated monocytes.||Endotoxin:|<1EU/ug (LAL)||Storage and Stability:|Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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