Chemokine (CXC motif) ligand 16, is a small cytokine belonging to the CXC chemokine family and signals through the CXCR6 receptor. CXCL16 is composed of its typical CXC chemokine domain, a mucin-like stalk, a transmembrane domain, and a cytoplasmic tail which allow CXCL16 to act as a cell surface bound molecule as well as a soluble chemokine. Human CXCL16 shares 70% amino acid sequence similarity with the mouse and CXCL16 expression is induced by IFN-gamma and TNF-alpha. CXCL16 is produced by dendritic cells in lymphoid organs, and by red pulp spleen cells. The expression of CXCL16 may attract lymphocytes during inflammation and assist some immune responses.||Source:|Recombinant protein corresponding to human CXCL16 expressed in E. coli.||Molecular Weight:|~10.4kD||Biological Activity:|The ED50 is 2.5-12ng/mL as determined by its ability to chemoattract activated lymphocytes (at a concentration of 100ng/mL).||Endotoxin:|<1EU/ug (LAL)||Storage and Stability:|Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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